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KMID : 0604520050310020197
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Journal of the Society of Cosmetic Scientists of Korea
2005 Volume.31 No. 2 p.197 ~ p.206
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Studies on the Antioxidative Effect of the Buckwheat (Fagopyrum esculentum Moench) Extract and its Protective Role against Cadmiun-mediated Stress
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Yoon Chang-Soon
Kim Nam-Kyung
Jeong Jae-Hyung
Choi Shin-Wook
Jang Kyung-Jin
Ko In-Young
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Abstract
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In the present study, the Chuncheon buckwheat extracts prepared from its seed coats, seeds and stems were used to determine anti-oxidative effects, the content of rutin and phytic acid, and the protective role against cadmium at the cellular level. futhermore, it was evaluated whether the buckwheat, mainly known as a healthy food source, might be applicable to functional cosmetics. Up to 100 {mu}g/mL of the extract was not toxic in HaCaT and B16F10 cell lines using MTT assay. The anti-oxidative capacity of superoxide radicals was shown in seed coats extracts > stem extracts=seed extracts. Although its content of rutin, known as one of effective anti-oxidants, mainly exists in the stem, any extract did not eliminate hydroxyl radicals. Phytic acid, known as a heavy metal-chelate agent, was highly concentrated in the stem. The Chuncheon buckwheat extract had 10% protective effect against the treatment of 50{mu}M cadmium at which 50% of HaCaT cells survived. Confocal laser scanning microscope revealed the intracellular generation of reactive oxygen species (ROS) by cadmium treatment. Finally, we identified that the stem extract had the most protective effect on the elimination of ROS
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KEYWORD
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buckwheat extracts, anti-oxidants, rutin, phytic acid, cadmium
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